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Analysis of l-tyrosine based on electrocatalytic oxidative reactions via screen-printed electrodes modified with multi-walled carbon nanotubes and nanosized titanium oxide (TiO2)

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Abstract

Method for electrochemical determination of l-tyrosine with screen-printed electrodes (SPE) modified with multi-walled CNT or CNT/TiO2 as sensing elements was used for the electroanalysis of l-tyrosine (Tyr). It was demonstrated that SPE/CNT and SPE/CNT/TiO2 exhibited high electrocatalytic activity and good analytical performance towards oxidation of l-tyrosine. The linear range of Tyr in human serum was 0.025 ÷ 1 mM with the correlation coefficient R2 = 0.97. Direct electrochemistry (without any mediator) of co-factor-free bovine serum albumin (BSA) and human serum albumin (HSA) was investigated by use of modified electrodes. Protein–ligand interactions based on the electrocatalytic oxidation of l-tyrosine during HSA interaction with hemin were analyzed by the change of peak height and oxidation peak area, corresponding to tyrosine oxidation accessibility.

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Acknowledgements

This investigation was performed within the framework of the Program for Basic Research of Russian State Academy of Sciences for 2013–2020.

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Authors and Affiliations

  1. Institute of Biomedical Chemistry, Pogodinskaya Street, 10, Moscow, 119121, Russia

    Victoria Shumyantseva, Tatiana Bulko, Alexey Kuzikov, Rami Masamrekh & Alexander Archakov

  2. Pirogov Russian National Research Medical University, Ostrovityanova Street, 1, Moscow, 117997, Russia

    Victoria Shumyantseva, Alexey Kuzikov, Rami Masamrekh & Alexander Archakov

Authors
  1. Victoria Shumyantseva
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  2. Tatiana Bulko
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  3. Alexey Kuzikov
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  4. Rami Masamrekh
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  5. Alexander Archakov
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Correspondence to Victoria Shumyantseva.

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Shumyantseva, V., Bulko, T., Kuzikov, A. et al. Analysis of l-tyrosine based on electrocatalytic oxidative reactions via screen-printed electrodes modified with multi-walled carbon nanotubes and nanosized titanium oxide (TiO2). Amino Acids 50, 823–829 (2018). https://doi.org/10.1007/s00726-018-2557-z

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  • DOI: https://doi.org/10.1007/s00726-018-2557-z

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